Dynamics of p38 MAP kinase inferred from a structural ensemble using PCA is compared to intrinsic dynamics of the protein modeled using ANM. See PCA of X-ray structures or Bioinformatics article for more details.
Results from comparative analysis of residue conservation, conformational mobility, and coevolutionary patterns for uracil-DNA glycosylase. See Mol Biol Evol article or Conservation and Coevolution Analysis for more details.
Comparative analysis of dynamics of drug target proteins and model systems from experiments (PCA) and theory (ANM). See the Protein Science article for details.
Comparative analysis of p38 MAP kinase dynamics from experiments (PCA), simulations (EDA), and theory (ANM). See the Protein Science article for details.
Animation shows HIV-1 reverse transcriptase functional motions calculated using anisotropic network model. Arrows and animations are generated using NMWiz VMD plugin. See NMWiz tutorial for usage examples.
You can make a quick protein representation in interactive sessions using showProtein() function.
NMWiz is designed for picturing normal modes easy. Image shows arrows from slowest three ANM modes for p38 MAP kinase centered at the origin. They indeed align with planes normal to each other.
NMWiz makes depicting elastic network models and protein motions predicted with them easy. Image shows ANM model for p38 MAP kinase and three slow ANM modes (below).
NMWiz can be used to comparative dynamics inferred from experimental datasets and predicted using theory.
Kinesin Eg5 druggable sites, including allosteric inhibitor binding site and and tubulin binding site, identified by simulations are shown. See our publication for details.
Sampling of the functional substates (inward-facing (IF) or outward-facing (OF), in closed (c) or open (o) forms) of LeuT using coMD simulations. See publication for details.
Energy landscape in the space of principal coordinates.
Outward-facing (OF) and inward-facing (IF) structures of GltPh show a large displacement of the core domains. See publication for details.
The second mode of the OF structure moves all three transport domains simultaneously through the membrane in a ‘lift-like’ motion. See publication for details.
The second mode of the IF structure moves all three transport domains simultaneously through the membrane in a ‘lift-like’ motion. See publication for details.
Deformability profile of ubiquitin (PDB code: 1UBI). Structure is automatically uploaded to VMD program where blue color shows regions which are mechanically more resistant to the external force.
Mean value of effective spring constant (calculated from mechanical stiffness matrix) with secondary structure of ubiquitin. Blue color indicates mechanically strong regions.
Mechanical Stiffness Map with effective force constant in a color bar (blue - strong regions, red - weak regions) for ubiquitin.
Workflow for GNM analysis of chromatin dynamics. See publication for details.
Covariance matrix of chromosome 17 of human B cells. Structural domains and CCDDs are identified and outlined. See publication for details.
3D Laplacian embedding of chromosome 17 loci using the first three principal modes. See ChromD tutorial for details.
Perturbation response scanning of the Hsp70 chaperone reveals interdomain allostery. See publication for details.
Perturbation response scanning of the AMPA-type glutamate receptor reveals sensors and effectors for allosteric signaling. See publication for details.
A more in-depth analysis of the PRS matrix reveals interdomain signaling in the AMPA receptor. See publication for details.
The left panels show the three softest GNM modes (blue lines) and their standard deviations (faint blue bands). Red and blue regions in the corresponding ribbon diagrams show regions moving in opposite directions. The right panel has the average cross-correlation matrix from the first 20 global modes (top) and its standard deviation (bottom).
Square fluctuations calculated from the top 5 global modes are shown for a number of LeuT fold family members, revealing similarities and subfamily- or conformation-dependent differences.
Type-I periplasmic binding protein domains are mapped onto the first two signature ANM modes. These domains, found in a range of proteins including bacterial solute carriers and eukaryotic receptors, have two lobes that undergo well-characterised conserved motions that are evident from comparison of structures. SignDy reveals such conserved dynamics.
SignDy analysis allows a comparison of the frequency dispersion of family members. The distribution of inverse eigenvalues is shown for the softest five modes for TIM barrel fold family.
v1.10.10 series come with new and improved sequence, structure, and dynamics analysis features. See release notes for details.
You can install ProDy and Evol using
pip install -U ProDy.
Please note that you may have to manually remove previous versions (<1.10.3) of
If you have pip, you can simply install ProDy as follows:
$ pip install prody
If you don't, you can manually download and extract tarball contents from PyPI website and run setup.py as follows:
$ tar -xzf ProDy-1.10.10.tar.gz $ cd ProDy-1.10.10 $ python setup.py build $ sudo python setup.py installIf you don’t have root access, see alternate installation schemes in Installing Python Modules.
Since version 1.10.3, ProDy does not provide a binary installer anymore. Instead, please install ProDy through pip:
$ pip install prody
Let us know any problems you might have by opening an issue at the tracker so that we can make ProDy better.